Composite
Part:BBa_K3409009:Design
Designed by: Lucie Pesenti Group: iGEM20_Ionis_Paris (2020-10-20)
Expression of receptor binding protein (gp37) on the outer membrane of Escherichia coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 3774
Illegal NheI site found at 3797 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 849
Illegal BglII site found at 2043
Illegal BglII site found at 3820 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 438
Illegal NgoMIV site found at 2223
Illegal AgeI site found at 1191
Illegal AgeI site found at 3875 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2426
Design Notes
Synthesis provided by Twist Bioscience
Source
This is a composite part that includes: Promoter (BBa_J23104), Ribosome binding site (RBS - BBa_B0030), Co-expressed lipoprotein with the outer membrane protein A (Lpp-OmpA - BBa_K1694002), Short linker (Gly-Gly-Ser-Gly) to fuse the proteins (BBa_K243004), TEV cleavage site (BBa_J18918), 10xHis-tag (BBa_K844000), Gene product (gp) 37 (BBa_K3409000), Double-terminator (BBa_B0015) Promoter medium (BBa_J23107), Ribosome binding site (RBS - BBa_B0032), chaperone protein gp38 (BBa_K3409001), chaperone protein 57A (BBa_K3409002), Terminator (BBa_B1005).