Composite

Part:BBa_K3409009:Design

Designed by: Lucie Pesenti   Group: iGEM20_Ionis_Paris   (2020-10-20)


Expression of receptor binding protein (gp37) on the outer membrane of Escherichia coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3774
    Illegal NheI site found at 3797
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 849
    Illegal BglII site found at 2043
    Illegal BglII site found at 3820
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 438
    Illegal NgoMIV site found at 2223
    Illegal AgeI site found at 1191
    Illegal AgeI site found at 3875
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2426


Design Notes

Synthesis provided by Twist Bioscience


Source

This is a composite part that includes: Promoter (BBa_J23104), Ribosome binding site (RBS - BBa_B0030), Co-expressed lipoprotein with the outer membrane protein A (Lpp-OmpA - BBa_K1694002), Short linker (Gly-Gly-Ser-Gly) to fuse the proteins (BBa_K243004), TEV cleavage site (BBa_J18918), 10xHis-tag (BBa_K844000), Gene product (gp) 37 (BBa_K3409000), Double-terminator (BBa_B0015) Promoter medium (BBa_J23107), Ribosome binding site (RBS - BBa_B0032), chaperone protein gp38 (BBa_K3409001), chaperone protein 57A (BBa_K3409002), Terminator (BBa_B1005).

References